MLPAO Clinical Chemistry Advanced Concepts

Question 1

Which method is used as the reference standard for measuring serum cholesterol?

Accepted Answer

Abell-Kendall method

Answer

Enzymatic cholesterol oxidase method

Answer

Liebermann-Burchard method

Answer

Gas chromatography-mass spectrometry

Question 2

What is the Friedewald equation used to calculate?

Accepted Answer

LDL cholesterol

Answer

HDL cholesterol

Answer

VLDL cholesterol

Answer

Total cholesterol

Question 3

Which analyte requires collection in a tube containing sodium fluoride to prevent glycolysis?

Accepted Answer

Glucose

Answer

Potassium

Answer

Calcium

Answer

Bilirubin

Question 4

What is the principle behind the enzymatic method for measuring serum triglycerides?

Accepted Answer

Lipase hydrolysis followed by glycerol kinase reaction

Answer

Direct spectrophotometric absorption of triglycerides

Answer

Saponification with potassium hydroxide

Answer

Thin-layer chromatography separation

Question 5

In therapeutic drug monitoring (TDM), what is a 'trough' level?

Accepted Answer

The minimum drug concentration just before the next dose

Answer

The maximum drug concentration after a dose

Answer

The average drug concentration over a dosing interval

Answer

The drug concentration at steady state

Question 6

Which condition is associated with elevated serum uric acid levels?

Accepted Answer

Gout

Answer

Hypothyroidism

Answer

Iron deficiency anemia

Answer

Addison disease