Processing Techniques Flashcards
7 cards from real CTBS practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 7 Processing Techniques flashcards as text
Which type of bone graft has both osteogenic AND osteoinductive AND osteoconductive properties?
Answer: Fresh autograft
Fresh autograft is the only graft type possessing all three bone healing properties: osteogenic (viable cells), osteoinductive (BMPs), and osteoconductive (scaffold), making it the gold standard.
In tissue bank processing, what is the purpose of performing a 'gram stain' during microbiological testing?
Answer: To differentiate between Gram-positive and Gram-negative bacteria for early contamination detection
Gram staining rapidly differentiates Gram-positive from Gram-negative bacteria based on cell wall composition, providing early indication of bacterial contamination during processing.
What is the primary concern with using high-dose gamma irradiation (> 25 kGy) to sterilize cortical bone allografts?
Answer: Significant reduction in compressive and torsional mechanical strength
High-dose gamma irradiation causes significant free radical-mediated damage to collagen cross-links, substantially reducing the compressive strength, fatigue resistance, and torsional properties of cortical bone.
During processing of amniotic membrane allografts, which property must be preserved to maintain its clinical utility as a wound cover?
Answer: Intact basement membrane and collagen matrix with growth factors
The intact basement membrane and collagen matrix containing growth factors (EGF, FGF, TGF-β) are essential for the amniotic membrane's anti-inflammatory, anti-scarring, and wound healing properties.
What does the term 'terminal sterilization' mean in tissue processing?
Answer: Sterilization applied to the final packaged product after all processing steps
Terminal sterilization refers to the application of a sterilization process (e.g., gamma irradiation, electron beam) to the tissue in its final, sealed packaging after all other processing steps are complete.
Which processing parameter is most critical to monitor and control during the cryopreservation of vascular allografts?
Answer: Cooling rate during controlled-rate freezing
Controlled cooling rate during cryopreservation is critical to minimize intracellular ice crystal formation, which would damage endothelial cell membranes and compromise graft viability.
What is the purpose of the 'secondary packaging' in tissue allograft processing?
Answer: To provide an additional barrier against contamination and allow for labeling
Secondary packaging provides an additional contamination barrier, structural protection, and serves as the surface for required labeling information while the primary package directly contacts the tissue.