BACE Microscopy and Imaging Techniques 2

Question 1

In confocal microscopy, what is the primary advantage over conventional fluorescence microscopy?

Accepted Answer

It eliminates out-of-focus fluorescence to produce sharper optical sections

Answer

It requires no fluorescent labels

Answer

It provides lower magnification

Answer

It uses visible light instead of laser light

Question 2

What is the correct procedure when switching from a lower to a higher power objective lens on a microscope?

Accepted Answer

Rotate the nosepiece while watching from the side, then use only the fine focus adjustment

Answer

Use the coarse focus adjustment to refocus the image

Answer

Increase the light intensity before switching objectives

Answer

Remove the slide and reposition it before switching

Question 3

Which component of a compound microscope controls the amount of light passing through the specimen?

Accepted Answer

Iris diaphragm

Answer

Condenser

Answer

Objective lens

Answer

Eyepiece

Question 4

What type of electron microscopy is used to examine the internal ultrastructure of thin-sectioned specimens?

Accepted Answer

Transmission electron microscopy (TEM)

Answer

Scanning electron microscopy (SEM)

Answer

Fluorescence electron microscopy

Answer

Confocal electron microscopy

Question 5

Why should microscope lenses be cleaned only with lens paper and lens cleaning solution?

Accepted Answer

To prevent scratching the lens coating and avoid contamination with oils from other materials

Answer

To make cleaning faster and more efficient

Answer

Because lens paper is sterile and prevents biological contamination

Answer

To apply additional optical coatings to the lens surface

Question 6

What is the function of the condenser in a compound light microscope?

Accepted Answer

To focus light onto the specimen to maximize illumination and resolution

Answer

To magnify the final image seen by the observer

Answer

To hold the objective lenses in position

Answer

To adjust the magnification of the eyepiece