Microscopy and Imaging Techniques Flashcards
6 cards from real BACE practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 6 Microscopy and Imaging Techniques flashcards as text
What is the total magnification when using a 10x eyepiece with a 40x objective lens on a compound microscope?
Answer: 400x
Total magnification is calculated by multiplying the eyepiece magnification (10x) by the objective lens magnification (40x), giving 400x.
Which type of microscopy uses fluorescent dyes or proteins to visualize specific cellular structures?
Answer: Fluorescence microscopy
Fluorescence microscopy uses fluorescent labels that emit light at specific wavelengths when excited, allowing visualization of tagged structures.
What is the purpose of immersion oil used with a 100x oil-immersion objective lens?
Answer: To increase resolution by eliminating refractive index differences between the lens and slide
Immersion oil has the same refractive index as glass, preventing light refraction at the lens-air interface and improving resolution.
Which microscopy technique is most suitable for observing live, unstained, transparent specimens?
Answer: Phase-contrast microscopy
Phase-contrast microscopy converts differences in light phase (caused by specimen thickness/density) into visible contrast without requiring staining.
What does the term 'resolution' refer to in microscopy?
Answer: The minimum distance between two points that can be distinguished as separate
Resolution is the ability of a microscope to distinguish two closely spaced points as separate objects; higher resolution means finer detail is visible.
Which staining technique uses crystal violet and iodine to differentiate bacteria into two groups based on cell wall structure?
Answer: Gram staining
Gram staining differentiates bacteria into gram-positive (purple) and gram-negative (pink) based on cell wall peptidoglycan content.