Laboratory Safety and Regulations Flashcards
6 cards from real BACE practice questions. Tap to flip, then mark Knew It or Still Learning — missed cards come back until you master them.
Read the first 6 Laboratory Safety and Regulations flashcards as text
What does BSL-2 (Biosafety Level 2) designation mean for a laboratory agent?
Answer: The agent poses moderate hazard to personnel and environment; risk groups include human pathogens causing non-severe disease with available treatment
BSL-2 agents present moderate hazard to personnel and environment and include organisms like Salmonella and hepatitis B virus; standard precautions plus BSC use and limited access are required.
Under OSHA 29 CFR 1910.1450 (Occupational Exposure to Hazardous Chemicals in Laboratories), what document is required in every academic or research laboratory?
Answer: A written Chemical Hygiene Plan (CHP) describing procedures and controls for chemical safety
29 CFR 1910.1450 requires laboratories to have a written Chemical Hygiene Plan (CHP) that outlines procedures, equipment, PPE, and work practices to protect employees from hazardous chemical exposures.
What is the 'Global Harmonized System' (GHS) for chemical classification, and what standard documents are associated with it?
Answer: An international framework harmonizing chemical hazard classification and communication worldwide implemented through standardized Safety Data Sheets and hazard labels
GHS is a UN system providing standardized criteria for classifying chemical hazards and communicating them through uniform SDSs (16 sections) and pictogram-based labels.
What is the purpose of a 'lockout/tagout' (LOTO) procedure in a laboratory setting?
Answer: To isolate and de-energize hazardous energy sources (electrical, mechanical, thermal) before equipment maintenance to prevent accidental startup
LOTO (OSHA 29 CFR 1910.147) ensures equipment is completely de-energized and cannot start unexpectedly while workers perform maintenance — preventing electrocution, crush injuries, and other energy-related accidents.
What does 'Risk Group 2' designation mean for a biological agent under NIH/CDC guidelines?
Answer: The agent is associated with human disease of varying severity; hazard to laboratory workers is moderate; risk to community is limited with effective prophylaxis/treatment available
Risk Group 2 agents cause human disease of moderate severity; they present limited risk to the community and have available treatments/prophylaxis — corresponding to BSL-2 containment.
When must a 'spill response kit' for biohazardous materials be used in a laboratory?
Answer: For any spill of potentially infectious material outside of a BSC or primary containment vessel
Any spill of potentially infectious material outside primary containment requires immediate decontamination using a biohazard spill kit — volume does not determine when a kit is needed.