BACE Bioprocessing and Fermentation 1

Question 1

What is a bioreactor primarily used for in biotechnology?

Accepted Answer

To provide a controlled environment for growing microorganisms or cells to produce biological products

Answer

To store biological samples at low temperatures

Answer

To separate proteins by molecular weight

Answer

To amplify DNA sequences

Question 2

What does the term 'sterile technique' mean in the context of bioprocessing?

Accepted Answer

Using methods that prevent contamination of cultures by unwanted microorganisms

Answer

Using high-temperature processes to kill all biological agents

Answer

Filtering all media through a 0.45-micron filter

Answer

Adding antibiotics to all growth media

Question 3

Which parameter is most critical to monitor during aerobic fermentation in a bioreactor?

Accepted Answer

Dissolved oxygen (DO) concentration

Answer

Light intensity inside the vessel

Answer

Atmospheric pressure outside the bioreactor

Answer

Color of the media

Question 4

What is the purpose of an autoclave in bioprocessing?

Accepted Answer

To sterilize media, equipment, and waste using steam under pressure

Answer

To centrifuge large volumes of culture broth

Answer

To filter sterile air into the bioreactor

Answer

To measure pH of growth media

Question 5

In batch fermentation, when does the 'stationary phase' occur?

Accepted Answer

When nutrient depletion causes the growth rate to equal the death rate, with no net change in cell number

Answer

When cells are first inoculated into fresh media

Answer

When cells are growing at their maximum rate

Answer

When the bioreactor is being cleaned between runs

Question 6

What is 'upstream processing' in bioprocessing?

Accepted Answer

All steps involved in growing and harvesting cells or microorganisms, including media preparation and fermentation

Answer

The purification of the final biological product

Answer

Quality control testing of the finished product

Answer

Formulation and packaging of the product