ASCP Tissue Processing 2

Question 1

During dehydration, tissues are passed through graded alcohols starting from what concentration?

Accepted Answer

70%

Answer

100%

Answer

95%

Answer

50%

Question 2

Which of the following is NOT a standard step in automated tissue processing?

Accepted Answer

Decalcification

Answer

Fixation

Answer

Dehydration

Answer

Clearing

Question 3

What temperature is paraffin wax typically maintained at during infiltration?

Accepted Answer

60°C

Answer

37°C

Answer

45°C

Answer

75°C

Question 4

What is the term for the process of surrounding embedded tissue with paraffin wax in a mold?

Accepted Answer

Embedding

Answer

Infiltration

Answer

Blocking

Answer

Casting

Question 5

Which fixative is preferred for electron microscopy due to superior ultrastructural preservation?

Accepted Answer

Glutaraldehyde

Answer

10% neutral buffered formalin

Answer

Zenker's fixative

Answer

Bouin's solution

Question 6

What is the primary purpose of a tissue processor's vacuum function?

Accepted Answer

To speed reagent infiltration into dense tissues

Answer

To prevent overheating of tissue

Answer

To remove excess paraffin

Answer

To filter contaminants from reagents