ASCP Special Stains and Histochemistry 3

Question 1

The Warthin-Starry stain is used to identify which organisms in tissue?

Accepted Answer

Spirochetes and Helicobacter pylori

Answer

Fungi

Answer

Acid-fast bacteria

Answer

Gram-negative rods only

Question 2

Which histochemical stain is used to demonstrate calcium deposits in tissue?

Accepted Answer

Von Kossa stain

Answer

Oil Red O

Answer

Alcian blue

Answer

Masson's trichrome

Question 3

What does Oil Red O stain identify in tissue sections?

Accepted Answer

Neutral lipids and triglycerides

Answer

Glycogen

Answer

Mucin

Answer

DNA

Question 4

In the Prussian blue stain, what positive control tissue is typically used?

Accepted Answer

Liver with hemosiderosis or spleen with iron deposits

Answer

Normal kidney

Answer

Lung with no pathology

Answer

Normal colon

Question 5

Which stain differentiates hyaline cartilage from fibrocartilage in musculoskeletal histology?

Accepted Answer

Safranin O/Fast Green

Answer

H&E only

Answer

PAS stain

Answer

Congo red

Question 6

What is the role of the mordant in a silver impregnation stain like GMS?

Accepted Answer

To sensitize tissue so it adsorbs silver ions

Answer

To reduce silver ions to metallic silver

Answer

To remove background silver deposits

Answer

To dehydrate sections before silver application