ASCP Hematoxylin and Eosin Staining 3

Question 1

In a well-stained H&E section, red blood cells (RBCs) should appear what color?

Accepted Answer

Bright red/orange

Answer

Blue-purple

Answer

Green

Answer

Colorless

Question 2

What is the purpose of rehydration after deparaffinization before H&E staining?

Accepted Answer

To remove residual xylene and prepare tissue for aqueous dyes

Answer

To fix tissue before staining

Answer

To remove excess eosin

Answer

To improve section adherence

Question 3

What does basophilic staining indicate in H&E histology?

Accepted Answer

Affinity for hematoxylin (basic dye)

Answer

Affinity for eosin (acidic dye)

Answer

Lack of staining

Answer

Affinity for both dyes equally

Question 4

What color does mucin typically stain in a routine H&E preparation?

Accepted Answer

Pale blue or clear

Answer

Deep blue-purple

Answer

Bright pink

Answer

Yellow

Question 5

What is the function of Scott's tap water (or ammonia water) in the H&E staining process?

Accepted Answer

Bluing agent to convert hematoxylin complex to blue

Answer

Dehydration step

Answer

Differentiation agent

Answer

Eosin rinse

Question 6

Which of the following is a quality control indicator for an H&E stained section?

Accepted Answer

Crisp blue nuclei with pink cytoplasm and no background staining

Answer

All cell types stain uniformly one color

Answer

Collagen stains blue

Answer

Nuclei stain red and cytoplasm stains blue