ASCP Hematoxylin and Eosin Staining 2

Question 1

What does 'over-differentiation' of hematoxylin produce in an H&E section?

Accepted Answer

Pale or unstained nuclei

Answer

Deep blue cytoplasm

Answer

Excessive eosin uptake

Answer

Green nuclear staining

Question 2

What type of hematoxylin is most commonly used in routine histology laboratories in the US?

Accepted Answer

Harris hematoxylin

Answer

Delafield's hematoxylin

Answer

Mayer's hematoxylin

Answer

Weigert's hematoxylin

Question 3

Which method of hematoxylin staining requires a differentiation step?

Accepted Answer

Regressive staining

Answer

Progressive staining

Answer

Vital staining

Answer

Metachromatic staining

Question 4

What is a common cause of eosin staining appearing too pale in H&E sections?

Accepted Answer

Insufficient eosin concentration or exhausted eosin solution

Answer

Over-fixation in formalin

Answer

Excessive differentiation with acid-alcohol

Answer

Over-dehydration during processing

Question 5

Which reagent is used to mount coverslips on H&E sections stained using an alcohol-based final dehydration?

Accepted Answer

Xylene-based mounting medium (e.g., Permount)

Answer

Aqueous mounting medium

Answer

Canada balsam only

Answer

Glycerin jelly

Question 6

What staining result indicates that sections were not completely deparaffinized before H&E staining?

Accepted Answer

Unstained or poorly stained areas with paraffin residue

Answer

Overly pale nuclei

Answer

Excessive eosin background

Answer

Green cytoplasm