SAP Drug & Alcohol Testing Procedures 1 — Questions and Answers
Question 1: What is the minimum urine specimen volume required for a valid DOT drug test collection?
- 30 mL
- 45 mL (Correct answer)
- 60 mL
- 90 mL
Correct answer: 45 mL
Under 49 CFR Part 40, a collector must obtain at least 45 mL of urine; anything less triggers the shy bladder protocol.
Question 2: What temperature range must a freshly voided urine specimen fall within to be considered valid under DOT regulations?
- 80–90°F (27–32°C)
- 85–95°F (29–35°C)
- 90–100°F (32–38°C) (Correct answer)
- 95–105°F (35–41°C)
Correct answer: 90–100°F (32–38°C)
49 CFR Part 40 requires the specimen temperature to be between 90°F and 100°F (32–38°C) when measured within 4 minutes of collection.
Question 3: Which of the following correctly lists the five drug categories tested in a standard DOT urine drug test?
- Marijuana, cocaine, opioids, PCP, amphetamines (Correct answer)
- Marijuana, cocaine, heroin, barbiturates, benzodiazepines
- Cocaine, opioids, amphetamines, MDMA, benzodiazepines
- Marijuana, opioids, barbiturates, benzodiazepines, PCP
Correct answer: Marijuana, cocaine, opioids, PCP, amphetamines
The DOT 5-panel test screens for marijuana (THC), cocaine, opioids, phencyclidine (PCP), and amphetamines, as specified in 49 CFR Part 40.
Question 4: At what blood alcohol concentration (BAC) level is a DOT-regulated employee considered to have an alcohol test violation?
- 0.02 or greater
- 0.04 or greater (Correct answer)
- 0.05 or greater
- 0.08 or greater
Correct answer: 0.04 or greater
Under 49 CFR Part 40, a confirmed BAC of 0.04 or greater constitutes an alcohol test violation and prohibits performance of safety-sensitive duties.
Question 5: Under DOT regulations, when is a urine drug test collection required to be conducted under direct observation?
- Only when the collector suspects specimen adulteration
- For all return-to-duty and follow-up tests (Correct answer)
- Whenever the employee requests it
- Only for pre-employment testing
Correct answer: For all return-to-duty and follow-up tests
49 CFR Part 40.67 mandates direct observation for all return-to-duty and follow-up collections to prevent tampering after a prior violation.
Question 6: What is the primary purpose of the Federal Chain of Custody Form (CCF) in DOT drug testing?
- To document the employee's informed consent to testing
- To track the specimen from collection through laboratory analysis and reporting (Correct answer)
- To record the MRO's final determination and disposition
- To authorize the SAP to begin the evaluation process
Correct answer: To track the specimen from collection through laboratory analysis and reporting
The CCF documents every transfer of custody, ensuring the integrity and traceability of the specimen from collection to laboratory result.
Question 7: How long must a certified laboratory retain a confirmed positive, adulterated, or substituted urine specimen under 49 CFR Part 40?
- 30 days
- 60 days
- 90 days
- At least 1 year (Correct answer)
Correct answer: At least 1 year
Laboratories must retain confirmed positive, adulterated, or substituted specimens for at least one year from the date the result was reported.
SAP Drug & Alcohol Testing Procedures 1
What is the minimum urine specimen volume required for a valid DOT drug test collection?