ALAT ALAT Research Procedures and Techniques Questions and Answers 2 — Questions and Answers
Question 1: Which anesthetic agent is commonly used as an injectable anesthetic combination in rodents for short procedures?
- Isoflurane and oxygen
- Ketamine and xylazine (Correct answer)
- Propofol and fentanyl
- Pentobarbital alone
Correct answer: Ketamine and xylazine
Ketamine/xylazine is a widely used injectable anesthetic combination in rodents for short to medium-length procedures.
Question 2: What is the correct method to confirm a rodent is under adequate anesthesia before beginning a procedure?
- Check that the animal is motionless
- Assess absence of response to a firm toe pinch (Correct answer)
- Verify the animal's eyes are closed
- Confirm the animal's heart rate is elevated
Correct answer: Assess absence of response to a firm toe pinch
Absence of a withdrawal response to a firm toe (pedal) pinch is the standard test for adequate anesthetic depth.
Question 3: Which of the following best describes the purpose of using an analgesic in post-procedural animal care?
- To induce sedation for easier handling
- To prevent infection at the surgical site
- To relieve pain and improve animal welfare (Correct answer)
- To accelerate wound healing
Correct answer: To relieve pain and improve animal welfare
Analgesics are administered post-procedurally to alleviate pain, supporting animal welfare and reducing stress-related confounds.
Question 4: What information should be recorded in a laboratory animal's procedure log?
- Only the date and type of procedure
- Date, procedure, dose, route, volume, and technician performing the procedure (Correct answer)
- Only abnormal findings during the procedure
- The researcher's name and grant number only
Correct answer: Date, procedure, dose, route, volume, and technician performing the procedure
Complete procedure records must include the date, procedure type, substance administered (dose, route, volume), and who performed it.
Question 5: When collecting a blood sample via cardiac puncture in a mouse, this procedure is typically performed:
- On a fully conscious restrained animal
- As a terminal procedure under anesthesia (Correct answer)
- On an animal in its home cage
- Only by a veterinarian, never a technician
Correct answer: As a terminal procedure under anesthesia
Cardiac puncture is a terminal procedure performed under deep anesthesia because it is not survivable.
Question 6: What is the primary reason for fasting an animal before certain experimental procedures?
- To reduce aggression during handling
- To prevent aspiration during anesthesia and standardize metabolic state (Correct answer)
- To decrease the animal's body weight for dosing calculations
- To make the animal easier to restrain
Correct answer: To prevent aspiration during anesthesia and standardize metabolic state
Fasting reduces aspiration risk during anesthesia and standardizes the metabolic state for reproducible experimental results.
ALAT ALAT Research Procedures and Techniques Questions and Answers 2
Which anesthetic agent is commonly used as an injectable anesthetic combination in rodents for short procedures?