ABPath Hematopathology 2 — Questions and Answers
Question 1: Auer rods on a peripheral blood smear are most characteristic of which condition?
- Acute myeloid leukemia (Correct answer)
- Chronic lymphocytic leukemia
- Hairy cell leukemia
- Multiple myeloma
Correct answer: Acute myeloid leukemia
Auer rods are azurophilic cytoplasmic inclusions of fused primary granules seen in myeloblasts of AML.
Question 2: The t(9;22) BCR-ABL1 fusion is the defining genetic lesion of which chronic myeloproliferative neoplasm?
- Chronic myeloid leukemia (Correct answer)
- Polycythemia vera
- Essential thrombocythemia
- Primary myelofibrosis
Correct answer: Chronic myeloid leukemia
The Philadelphia chromosome t(9;22) producing BCR-ABL1 defines CML.
Question 3: A CD5-positive, CD23-positive small B-cell lymphoma with smudge cells on smear is most consistent with:
- Chronic lymphocytic leukemia/small lymphocytic lymphoma (Correct answer)
- Mantle cell lymphoma
- Follicular lymphoma
- Burkitt lymphoma
Correct answer: Chronic lymphocytic leukemia/small lymphocytic lymphoma
CLL/SLL is CD5+/CD23+ and classically shows smudge cells on the peripheral smear.
Question 4: Reed-Sternberg cells are the diagnostic hallmark of which lymphoma?
- Classic Hodgkin lymphoma (Correct answer)
- Diffuse large B-cell lymphoma
- Marginal zone lymphoma
- Anaplastic large cell lymphoma
Correct answer: Classic Hodgkin lymphoma
Binucleate Reed-Sternberg cells (CD15+/CD30+) define classic Hodgkin lymphoma.
Question 5: Which stain is used to demonstrate iron stores in bone marrow aspirate smears?
- Prussian blue (Perls) (Correct answer)
- Periodic acid-Schiff
- Myeloperoxidase
- Reticulin silver stain
Correct answer: Prussian blue (Perls)
Prussian blue (Perls) reaction highlights hemosiderin iron in marrow.
Question 6: The JAK2 V617F mutation is most frequently associated with which myeloproliferative neoplasm?
- Polycythemia vera (Correct answer)
- Chronic myeloid leukemia
- Acute promyelocytic leukemia
- Chronic lymphocytic leukemia
Correct answer: Polycythemia vera
JAK2 V617F is present in nearly all cases of polycythemia vera.
Question 7: A serum protein electrophoresis showing a monoclonal M-spike with marrow plasmacytosis and lytic bone lesions indicates:
- Multiple myeloma (Correct answer)
- Waldenstrom macroglobulinemia
- Reactive plasmacytosis
- Monoclonal gammopathy of undetermined significance
Correct answer: Multiple myeloma
M-spike with clonal plasma cells, lytic lesions, and end-organ damage defines multiple myeloma.
ABPath Hematopathology 2
Auer rods on a peripheral blood smear are most characteristic of which condition?