ABPath Blood Banking and Transfusion 2 — Questions and Answers
Question 1: A patient's serum reacts with all reagent red cells in an antibody panel including the autocontrol. What is the most likely cause?
- A warm autoantibody (Correct answer)
- Anti-K alloantibody
- A single low-frequency antibody
- Rouleaux only
Correct answer: A warm autoantibody
Pan-reactivity with a positive autocontrol typically indicates a warm autoantibody.
Question 2: Which blood group system antigen is destroyed by treating red cells with proteolytic enzymes like ficin?
- Fya (Duffy) (Correct answer)
- Jka (Kidd)
- K (Kell)
- E (Rh)
Correct answer: Fya (Duffy)
Duffy antigens (Fya/Fyb) are denatured by proteolytic enzymes such as ficin and papain.
Question 3: A group O mother delivers a group A infant with a positive DAT and jaundice at birth. What is the most likely diagnosis?
- ABO hemolytic disease of the newborn (Correct answer)
- Rh hemolytic disease
- Hereditary spherocytosis
- G6PD deficiency
Correct answer: ABO hemolytic disease of the newborn
Group O mothers produce IgG anti-A,B that crosses the placenta causing ABO HDN.
Question 4: What is the minimum hemoglobin concentration typically required for an allogeneic whole blood donor in the US?
- 12.5 g/dL (Correct answer)
- 10.0 g/dL
- 13.0 g/dL
- 11.0 g/dL
Correct answer: 12.5 g/dL
US regulations require a minimum hemoglobin of 12.5 g/dL for allogeneic donors.
Question 5: A febrile nonhemolytic transfusion reaction is most commonly caused by which mechanism?
- Cytokines from donor leukocytes and recipient antibodies to leukocytes (Correct answer)
- ABO incompatibility
- IgA deficiency
- Bacterial contamination
Correct answer: Cytokines from donor leukocytes and recipient antibodies to leukocytes
FNHTRs result from accumulated cytokines and recipient anti-leukocyte antibodies.
Question 6: Which component is the treatment of choice for fibrinogen replacement in a bleeding patient with hypofibrinogenemia?
- Cryoprecipitate (Correct answer)
- Packed red blood cells
- Platelets
- Albumin
Correct answer: Cryoprecipitate
Cryoprecipitate is rich in fibrinogen and is used to correct hypofibrinogenemia.
Question 7: At what temperature must red blood cell units be stored during refrigerated storage?
- 1 to 6°C (Correct answer)
- 20 to 24°C
- -18°C or colder
- 10 to 15°C
Correct answer: 1 to 6°C
RBC units are stored refrigerated at 1 to 6°C.
ABPath Blood Banking and Transfusion 2
A patient's serum reacts with all reagent red cells in an antibody panel including the autocontrol.
What is the most likely cause?